Protective effect of catechin from black tea in an in-vitro model of myocardial ischemic reperfusion injury

Verma, R and Das, M and Mohamed Saleem, T.S and Kumar, L and Gauthaman, K (2014) Protective effect of catechin from black tea in an in-vitro model of myocardial ischemic reperfusion injury. Journal of Pharmacy Research, 8 (1). pp. 1-6. ISSN 0974-6943

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Abstract

Present study was designed to compare cardio-protective activity of crude aqueous extract and catechin fractions of black tea on the oxidative stress model of myocardial ischemic reperfusion injury in rat. Method: Black tea extract and catechin fraction were administered to male Wister rats weighing between 200-250 g in three different doses (n=6), by gastric gavage at a dose of 500 mg/kg body weight (BT1), 1000 mg/kg body weight (BT2), 1500 mg/kg body weight (BT3) and catechin fraction at a dose of 10 mg/kg body weight (CT1), 20 mg/kg body weight (CT2), 30 mg/kg body weight (CT3) daily for thirty days. Rat’s heart was subjected to in vitro global ischemic reperfusion injury (5 min perfusion, 9 min no flow and 12 min reperfusion). Biochemical parameters like TBARS, SOD, GSH and catalase were measured in treated groups at basal level and in in vitro ischemic reperfusion injury of the treated groups. Results: Pre-treatment with the black tea crude extract on the dose of both 500 & 1000 mg/kg and catechin 10, 20 & 30 mg/kg has a definite, dose-dependent modulatory effect on the antioxidant milieu of heart. CT2IR treated group offered better cardio-protection when subjected to IR injury. No definite pattern of change in the level of TBARS and endogenous antioxidants was observed. Hence these modulatory actions seem to be enzyme-specific. Conclusion: From these findings it has been concluded that Catechin fraction in a smaller dose (20 mg/kg) shows significant cardioprotective activity when compared with other doses and parent black tea extract. Further research is required to find out the mechanism of action of Catechin for cardioprotection.

Item Type: Article
Subjects: Medicine and Surgery
Pharmacy
Biology
Divisions: College of Pharmacy > Pharmacy
Depositing User: GAUTHAMAN Karunakaran
Date Deposited: 05 Mar 2017 21:17
Last Modified: 05 Mar 2017 21:17
URI: http://eprints.kku.edu.sa/id/eprint/478

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