Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)

Khaled M. Alakhali, K (2013) Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS). Journal of Clinical and Diagnostic Research, 7 (12). pp. 2739-2743. ISSN 6261-3748

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Abstract

Introduction: The Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS) for determination simvastatin in human plasma has been developed after extraction by by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard. Material and Methods: The mobile phase consisting of mixture of acetonitrile and water (75/25%, v/v) 500μL/min by separated the solutes on a C18 column. D iscussion: The lower limit of quantitation of 0.25 ng/mL was achieved when the calibration curve was linear from 0.25-50 ng/mL. The entire run time for analysis was only 6 min. The quantitation in the selective reaction monitoring (SRM) in positive ion mode, the daughter ions m/z 325 for simvastatin and m/z 285 for lovastatin were used. The Parent ions in positive ion mode were m/z 441.3 for simvastatin and m/z 405.1 for lovastatin. The intra-day coefficients of variation were less than 14% while the inter-day coefficients of variation were less than 10%. Conclusion: The LC-MS-MS detection is sensitive due to its capability to eliminate interferences from endogenous components.

Item Type: Article
Subjects: Pharmacy
Divisions: College of Pharmacy > Pharmacy
Depositing User: KHALED ALAKHALI
Date Deposited: 08 Jun 2017 10:53
Last Modified: 08 Jun 2017 10:53
URI: http://eprints.kku.edu.sa/id/eprint/975

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