VALIDATION METHOD FOR MEASURING SIMVASTATIN IN HUMAN PLASMA BY HPLC-UV AND ITS APPLICATION IN STUDY SIMVASTATIN STABILITY IN PLASMA AND WORKING SOLUTION

KHALED. M. ALAKHALI, K (2014) VALIDATION METHOD FOR MEASURING SIMVASTATIN IN HUMAN PLASMA BY HPLC-UV AND ITS APPLICATION IN STUDY SIMVASTATIN STABILITY IN PLASMA AND WORKING SOLUTION. Asian J Pharm Clin Res, 7 (2). pp. 131-133. ISSN 0974-2441

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Abstract

Objective: The aim of this study by HPLC-UV method for determination of simvastatin in human plasma has been developed, after extraction by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard. Methodology: Solutes are separated on a C18 column with mobile phase consist a mixture of acetonitrile-water (51/49%, v/v) mL/min and UV detection at 238 nm. The calibration curve was linear from 20 to 1000 ng/mL (r = 0.9996). Result: The intra-day coefficients of variation were less than 6.00% and the accuracies were between 97.52 and 104.80% for human control plasma containing 50, 200 and 500 ng/mL of simvastatin, respectively. The inter-day coefficients of variation were less than 9.00% and the accuracies were between 101.65 and 105.16% for human control plasma containing 50, 200 and 500 ng/mL of simvastatin, respectively. The entire run time for analysis was only 11 min. The limit of quantitation of 20 ng/mL was achieved. Conclusion: The stability studies of simvastatin in human plasma for two months at -850C, in acetonitrile and water for one month at 40C, did not show any significant degradation.

Item Type: Article
Subjects: Pharmacy
Divisions: College of Pharmacy > Pharmacy
Depositing User: KHALED ALAKHALI
Date Deposited: 23 Aug 2017 08:56
Last Modified: 23 Aug 2017 08:56
URI: http://eprints.kku.edu.sa/id/eprint/978

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