Development of Sudan III based lipid staining method and pharmacological evaluation of lipid droplets role in cancer cells adaptation to serum starvation

Abdallah, Qasem and El-Deeb, Ibrahim and ALMatani, Mohammed F and ALRashed, Amjad and AlKherish, Omar and ALMarri, Khaled (2017) Development of Sudan III based lipid staining method and pharmacological evaluation of lipid droplets role in cancer cells adaptation to serum starvation. Other thesis, Taif University.

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Abstract

Sudan III is a red color reagent that has the ability to bind lipids and thus is used to determine the fat (lipid) level in plants, food and feces. In our study we develop a method to use Sudan III as a selective stain for lipid droplets in colorectal cancer cells. Using the optimized Sudan III method we have also investigated the role of lipid droplets in cancer cell adaptation to serum starvation. Inhibiting phospholipase A2 by betamethasone has decreased the formation of lipid droplets as well as caused growth inhibition in serum-starved cell and not nutrient supplemented cells. This further emphasis on the importance of lipid droplets and phospholipase A2 enzyme in cellular adaptation of cancer cells to starvation.

Item Type: Thesis (Other)
Subjects: Pharmaceutical Sciences
Pharmacist
Pharmacy
Divisions: College of Pharmacy > Pharmacy
Depositing User: Ù�ح Ø¢Ù�
Date Deposited: 29 Nov 2017 03:27
Last Modified: 29 Nov 2017 03:27
URI: http://eprints.kku.edu.sa/id/eprint/1803

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