MSCs ameliorates DPN induced cellular pathology via [Ca2+ ]i homeostasis and scavenging the pro-inflammatory cytokines.

Chandramoorthy, HC and Bin-Jaliah, I and Karari, H and Rajagopalan, P and Ahmed Shariff, ME and Al-Hakami, A and Al-Humayad, SM and Baptain, FA and Ahmed, HS and Yassin, H and Haidara, MA (2017) MSCs ameliorates DPN induced cellular pathology via [Ca2+ ]i homeostasis and scavenging the pro-inflammatory cytokines. MSCs ameliorates DPN induced cellular pathology via [Ca2+]i homeostasis and scavenging the pro-inflammatory cytokines. pp. 1331-1340.

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Official URL | رابط موقع المجلة: http://onlinelibrary.wiley.com/doi/10.1002/jcp.260...

Abstract|ملخص البحث

The MSCs of various origins are known to ameliorate or modulate cell survival strategies. We investigated, whether UCB MSCs could improve the survival of the human neuronal cells and/or fibroblast assaulted with DPN sera. The results showed, the co-culture of UCB MSCs with human neuronal cells and/or fibroblasts could effectively scavenge the pro-inflammatory cytokines TNF-α, IL-1β, IFN-ɤ and IL - 12 and control the pro-apoptotic expression of p53/Bax. Further co-culture of UCB MSCs have shown to induce anti-inflammatory cytokines like IL-4, IL-10 and TGF-β and anti-apoptotic Bclxl/Bcl2 expression in the DPN sera stressed cells. Amelioration of elevated [Ca2+ ]i and cROS, the portent behind the NFκB/Caspase-3 mediated inflammation in DPN rescued the cells from apoptosis. The results of systemic administration of BM MSCs improved DPN pathology in rat as extrapolated from human cell model. The BM MSCs ameliorated prolonged distal motor latency (control: 0.70 ± 0.06, DPN: 1.29 ± 0.13 m/s DPN + BM MSCs: 0.89 ± 0.02 m/s, p < 0.05) and lowered high amplitude of compound muscle action potentials (CMAPs) (control: 12.36 ± 0.41, DPN: 7.52 ± 0.61 mV, DPN + MSCs: 8.79 ± 0.53 mV, p < 0.05), while slowly restoring the plasma glucose levels. Together, all these results showed that administration of BM or UCB MSCs improved the DPN via ameliorating pro-inflammatory cytokine signaling and [Ca2+ ]i homeostasis.

Item Type|تصنيف النتاج العلمي: Article| منشور علمي
Subjects | مجال موضوع النشر: Medical laboratory
Medical-Surgery Nursing
Medicine and Surgery
Pharmaceutical Sciences
Biology
Divisions | الكلية: College of Medicine > Medicine and Surgery
Depositing User: Ahmed Al Hakami
Date Deposited: 08 Mar 2018 13:32
Last Modified: 08 Mar 2018 13:33
URI: http://eprints.kku.edu.sa/id/eprint/3156

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