Simplified analysis of HRRT brain PET data with [11C]DASB

Assiri, Rajeh (2016) Simplified analysis of HRRT brain PET data with [11C]DASB. Masters thesis, The University of Manchester.

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Abstract|ملخص البحث

Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter that plays a pivotal role in the pathophysiology of mood disorders and in the regulation of emotion. In the central nervous system, the serotonin transporter (SERT) or serotonin reuptake site plays a key role in the regulation of the serotonin levels in the synaptic cleft. Non-invasive imaging with positron emission tomography (PET) has widely been used to measure in vivo SERT binding. [11C]DASB is a radiotracer for SERT with a higher ratio of specific binding to non- specific binding than earlier compounds used for single-photon emission computed tomography (SPECT) or PET. Full quantification of SERT availability with [11C]DASB PET requires an arterial plasma input function [1]. However, this can be technically challenging and, therefore, some [11C]DASB studies have relied on simplified quantification methods with reference tissue input. In a previous study, 12 healthy males were scanned twice with [11C]DASB on the High Resolution Research Tomograph (HRRT), after receiving placebo or treatment with the centrally acting monoamine reuptake inhibitor sibutramine. The objective of this project is to use a range of simplified methods to re- analyse this data set, both with region-of-interest tissue time activity curves and parametric mapping methods. This required the regularisation of the dynamic PET date prior to kinetic analysis to reduce noise-induced bias in the parameter estimates. Particular attention was paid to regional and between subject variability of occupancy as the specific binding of [11C]DASB may not be high enough in some areas of the brain to permit reliable quantification. The overall outcome of the project established the scope and limitations of simplified analysis methods for clinical [11C]DASB studies acquired on the HRRT

Item Type|تصنيف النتاج العلمي: Thesis | رسالة بحثية (Masters)
Subjects | مجال موضوع النشر: Chemistry
Pharmaceutical Sciences
Radiological Sciences
Divisions | الكلية: College of Applied Medical Sciences > Radiological Sciences
Depositing User: Mr. Rajeh Assiri
Date Deposited: 27 Dec 2017 03:31
Last Modified: 27 Dec 2017 03:31

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