Resveratrol reverses cadmium chloride-induced testicular damage and subfertility by downregulating p53 and Bax and upregulating gonadotropins and Bcl-2 gene expression

Eleawa, Samy and Alkhateeb, Mahmoud and Alhashem, Fahaid and Bin-Jaliah, Ismaeel and Sakr, Hussein and Elrefaey, Hesham and Elkarib, Abbas and Alessa, Riyad and Haidara, Mohammad and Shatoor, Abdullah and Khalil, Mohammad (2014) Resveratrol reverses cadmium chloride-induced testicular damage and subfertility by downregulating p53 and Bax and upregulating gonadotropins and Bcl-2 gene expression. Journal of Reproduction and Development, 60 (2). pp. 115-127. ISSN 0916-8818

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Official URL | رابط موقع المجلة: https://www.jstage.jst.go.jp/article/jrd/60/2/60_2...

Abstract|ملخص البحث

This study was performed to investigate the protective and therapeutic effects of resveratrol (RES) against CdCl2-induced toxicity in rat testes. Seven experimental groups of adult male rats were formulated as follows: A) controls+NS, B) control+vehicle (saline solution of hydroxypropyl cyclodextrin), C) RES treated, D) CdCl2+NS, E) CdCl2+vehicle, F) RES followed by CdCl2 and M) CdCl2 followed by RES. At the end of the protocol, serum levels of FSH, LH and testosterone were measured in all groups, and testicular levels of TBARS and superoxide dismutase (SOD) activity were measured. Epididymal semen analysis was performed, and testicular expression of Bcl-2, p53 and Bax was assessed by RT-PCR. Also, histopathological changes of the testes were examined microscopically. Administration of RES before or after cadmium chloride in rats improved semen parameters including count, motility, daily sperm production and morphology, increased serum concentrations of gonadotropins and testosterone, decreased testicular lipid peroxidation and increased SOD activity. RES not only attenuated cadmium chloride-induced testicular histopathology but was also able to protect against the onset of cadmium chloride testicular toxicity. Cadmium chloride downregulated the anti-apoptotic gene Bcl2 and upregulated the expression of pro-apoptotic genes p53 and Bax. Resveratrol protected against and partially reversed cadmium chloride testicular toxicity via upregulation of Bcl2 and downregulation of p53 and Bax gene expression. The antioxidant activity of RES protects against cadmium chloride testicular toxicity and partially reverses its effect via upregulation of BCl2 and downregulation of p53 and Bax expression.

Item Type|تصنيف النتاج العلمي: Article| منشور علمي
Subjects | مجال موضوع النشر: Medicine and Surgery
Biology
Divisions | الكلية: College of Medicine > Physiology
Depositing User: Prof. Ismaeel Bin-Jaliah
Date Deposited: 28 Dec 2018 18:11
Last Modified: 28 Dec 2018 18:11
URI: http://eprints.kku.edu.sa/id/eprint/4140

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