Partial Purification of two Novel Variants of Bacteriocins Produced by Lactic Acid Bacteria

negm, sally and Abdel-Shafi, Seham and Mohammadi, Abdul-Raouf and Enan, Gamal (2014) Partial Purification of two Novel Variants of Bacteriocins Produced by Lactic Acid Bacteria. Global Veterinaria, 13 (4). pp. 583-589. ISSN 1992-6197


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Abstract|ملخص البحث

Abstract: The work employed described the partial purification and physicochemical characterization of two bacteriocins produced by two strains of lactic acid bacteria (LAB) namely: Lactococcus lactis subsp. Lactis Z ( 11 L. lactis Z ) and 11 Lactobacillus delbrueckii subsp. Bulgaricus Z ( 55 Lb. bulgaricus Z ); both strains were 55 isolated from Arabian yoghurt. The two bacteriocins were purified by two-stepsprotocol including ammonium sulphate precipitation and ion-exchange chromatography across sephadex G 200-50 column. The two bacteriocins were precipitated ideally at 50-60% ammonium sulphate saturation levels in the pH range 5.0-6.0. The specific activity of L. lactis Z bacteriocin and Lb. bulgaricus Z bacteriocin was increased by102 times; 11 55 12.7 times respectively. Bacteriocin activities showed further increase by ion-exchange chromatography asabout 383 fold-increase and 170 fold-increase in specific bacteriocin activity was reported for L. lactis Z bacteriocin 11 and Lb. bulgaricus Z bacteriocins respectively. SDS-PAGE analysis revealed that 55 L. lactis Z bacteriocin and 11 Lb. bulgaricus Z bacteriocin had a molecular weight of 3.5 and 5.0kDa respectively. Amino acid analysis of 55 two bacteriocins showed that L. lactis Z bacteriocin is alantibiotic bacteriocin and 11 Lb. bulgaricus Z55 bacteriocin is a non-lantibiotic bacteriocin. Consequently the former designated lacticin Z andwas classified 11 as a member within class I bacteriocins and the latter was designated bulgaricin Z and was classified as a 55 novel variant within class IIa pediocin- like bacteriocins.

Item Type|تصنيف النتاج العلمي: Article| منشور علمي
Subjects | مجال موضوع النشر: Medical laboratory
Divisions | الكلية: College of Applied Medical Sciences - Mahyal Aseer > Nursing
Depositing User: Sally Nasr
Date Deposited: 23 Jan 2019 06:54
Last Modified: 23 Jan 2019 06:54

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