Anti-cancer action of a new recombinant lectin produced from Acacia species

Patel, Ayyub and Hafez, Elsayed and Elsaid, Fahmy and Amanullah, Mohammed (2014) Anti-cancer action of a new recombinant lectin produced from Acacia species. Journal of Medicine and Medical Sciences, 5 (1). pp. 1-11.

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Official URL | رابط موقع المجلة: http:/dx.doi.org/10.14303/jmms.2014.018

Abstract|ملخص البحث

The role of a new lectin protein from Acacia species found in Aseer region of Kingdom of Saudi Arabia, as anti-cancer agent on four cell lines viz. HepG-2 (Hepatocellular carcinoma), MCF-7 (Breast cancer), HCT 116 (colon Cancer) and HEP-2 (Larynx cancer) was evaluated. Acacia seyal del, was collected from Aseer region of the Kingdom of Saudi Arabia. The plant tissue was subjected to RNA extraction, the extracted RNA was used for lectin gene amplification using specific primers. Cloning, subcloning of the Acacia 400bp gene was carried out and in vitro transcription, combined with protein purification was undertaken. The cytotoxicity of the recombinant lectin was performed on HepG-2 (Hepatocellular carcinoma), MCF-7 (Breast cancer), HCT 116 (colon Cancer) and HEP-2 (Larynx cancer). Our study resulted in the observation of two amplicones with molecular sizes of 800 and 400bp. The 400bp amplicone was excised from the agrose gel, purified and sequenced. The sequence analysis revealed that the lectin gene isolated from Acacia seyal del showed similarity with Lotus japonicus nod factor binding lectin gene with an identity of 95%. The purified lectin exhibited a significant cytotoxic effect on MCF7cell. The IC50 value for HepG-2 cells was 100 μg/ml and for MCF7 was 250 μg/ml interposed from linear regression curve. Thus we conclude that Acacia lectin has anticancer action at different concentrations especially at a high dose 100 μg/ml.

Item Type|تصنيف النتاج العلمي: Article| منشور علمي
Subjects | مجال موضوع النشر: Chemistry
Medicine and Surgery
Biology
Divisions | الكلية: College of Medicine > Clinical Biochemistry
Depositing User: Dr Ayyub Ali Patel
Date Deposited: 22 Jan 2019 07:45
Last Modified: 22 Jan 2019 07:45
URI: http://eprints.kku.edu.sa/id/eprint/4252

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